Anemia merupakan masalah kesehatan yang ditandai dengan penurunan jumlah eritrosit
atau kadar hemoglobin. Bayam merah (Amaranthus tricolor L.) mengandung zat besi,
vitamin C, asam folat, dan flavonoid yang berpotensi mendukung proses eritropoiesis.
Penelitian ini bertujuan untuk mengetahui pengaruh pemberian suspensi bayam merah
terhadap jumlah retikulosit pada tikus putih (Rattus norvegicus) model anemia serta
menentukan dosis optimum. Penelitian ini merupakan penelitian eksperimental laboratorik
menggunakan 25 ekor tikus putih jantan galur Wistar yang dibagi menjadi lima kelompok,
yaitu kelompok normal, kelompok negatif, dan tiga kelompok perlakuan yang diberi
suspensi bayam merah dengan dosis 0,2 g/kgBB, 0,4 g/kgBB, dan 0,6 g/kgBB. Model
anemia diinduksi menggunakan phenylhydrazine (PHZ) selama 2 hari dan deferiprone
selama 7 hari, kemudian diberikan suspensi bayam merah selama 14 hari. Pemeriksaan
jumlah retikulosit dilakukan secara manual menggunakan pewarna Brilliant Cresyl Blue
(BCB). Data dianalisis menggunakan uji One Way ANOVA. Hasil penelitian
menunjukkan rata-rata jumlah retikulosit pada kelompok normal, negatif, dosis 0,2
g/kgBB, dosis 0,4 g/kgBB, dan dosis 0,6 g/kgBB berturut-turut sebesar 1,54%; 2,04%;
1,54%; 1,26%; dan 0,92%. Hasil uji One Way ANOVA menunjukkan terdapat perbedaan
yang bermakna antar kelompok (p = 0,001). Berdasarkan kedekatan nilai rata-rata
retikulosit terhadap kelompok normal, dosis 0,2 g/kgBB merupakan dosis optimum
Kesimpulannya, pemberian suspensi bayam merah berpengaruh terhadap jumlah
retikulosit pada tikus putih (Rattus norvegicus) model anemia, dengan dosis optimum
sebesar 0,2 g/kgBB berdasarkan parameter jumlah retikulosit.

Anemia is a health condition characterized by a decrease in the number of erythrocytes or
hemoglobin levels. Red spinach (Amaranthus tricolor L.) contains iron, vitamin C, folic
acid, and flavonoids that have the potential to support the process of erythropoiesis. This
study aimed to determine the effect of red spinach suspension administration on
reticulocyte count in an anemic rat model (Rattus norvegicus) and to determine the
optimum dose. This study was a laboratory experimental study using 25 male Wistar rats
divided into five groups: a normal group, a negative control group, and three treatment
groups receiving red spinach suspension at doses of 0.2 g/kg body weight, 0.4 g/kg body
weight, and 0.6 g/kg body weight. Anemia was induced using phenylhydrazine (PHZ) for
2 days and deferiprone for 7 days, followed by the administration of red spinach suspension
for 14 days. Reticulocyte counts were determined manually using Brilliant Cresyl Blue
(BCB) staining. The data were analyzed using the One-Way ANOVA test. The results
showed that the mean reticulocyte counts in the normal group, negative control group, 0.2
g/kg body weight group, 0.4 g/kg body weight group, and 0.6 g/kg body weight group were
1.54%, 2.04%, 1.54%, 1.26%, and 0.92%, respectively. The One-Way ANOVA test
demonstrated a statistically significant difference among the groups (p = 0.001). Based on
the similarity of the mean reticulocyte count to that of the normal group, the optimum dose
was 0.2 g/kg body weight. In conclusion, the administration of red spinach suspension
significantly affected the reticulocyte count in an anemic rat model (Rattus norvegicus),
with the optimum dose being 0.2 g/kg body weight based on the reticulocyte count
parameter.