Salmonella typhimurium merupakan salah satu bakteri penyebab salmonellosis yang masih menjadi masalah kesehatan masyarakat. Peningkatan resistensi antibiotik mendorong pengembangan alternatif antimikroba, salah satunya bakteriosin yang di produksi oleh Lactobacillus plantarum. Produksi bakteriosin diduga dapat ditingkatkan melalui suplementasi bahan yang mengandung komponen prebiotik, seperti jamur kuping (Auricularia auricula-judae) yang kaya akan polisakarida dan β-glukan. Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi ekstrak etanol jamur kuping terhadap aktivitas antimikroba bakteriosin L. plantarum terhadap S. typhimurium.
Penelitian ini menggunakan metode eksperimental. Kultur L. plantarum disuplementasi ekstrak etanol jamur kuping pada konsentrasi 0,1%; 0,2%; 0,4%; 0,8%; dan 1,6%, kemudian dilakukan isolasi bakteriosin dan pengujian aktivitas antimikroba menggunakan metode difusi sumuran. Data dianalisis secara deskriptif, dilanjutkan uji One Way ANOVA dan Post Hoc-LSD.
Hasil Penelitian menunjukkan bahwa seluruh kelompok perlakuan mampu menghambat pertumbuhan S. typhimurium. Diameter zona hambat terbesar pada kelompok perlakuan diperoleh pada konsentrasi 0,8% sebesar 10,25 ± 0,95 mm, sedangkan kontrol positif menghasilkan diameter zona hambat sebesar 11,25 ± 0,95 mm.
Hasil uji One Way ANOVA menunjukkan terdapat perbedaan yang signifikan antar kelompok perlakuan (p=0,049). Suplementasi ekstrak etanol jamur kuping belum mampu mendukung aktivitas antimikroba bakteriosin yang lebih tinggi dibandingkan kontrol positif.

Kata Kunci: A. auricula-judae, bakteriosin, L. plantarum, S.typhimurium, zona hambat.
Kepustakaan : 36 2015 - 2025

Salmonella typhimurium is one of the bacteria that cause salmonellosis, which remains a public health concern. The rise in antibiotic resistance has driven the development of alternative antimicrobials, one of which is bacteriocin produced by Lactobacillus plantarum. Bacteriocin production is thought to be enhanced by supplementing the culture with prebiotic-rich materials, such as the wood ear mushroom (Auricularia auricula-judae), which is rich in polysaccharides and β-glucans. This study aimed to determine the effect of supplementing L. plantarum cultures with ethanol extract of the wood ear mushroom on the antimicrobial activity of the bacteriocin against S. typhimurium.
This study employed an experimental design. L. plantarum cultures were supplemented with ethanol extract of the wood ear mushroom at concentrations of 0.1%, 0.2%, 0.4%, 0.8%, and 1.6%; bacteriocin was then isolated, and antimicrobial activity was tested using the well diffusion method. The data were analyzed descriptively, followed by a one-way ANOVA and a post hoc LSD test.
The results showed that all treatment groups were able to inhibit the growth of S. typhimurium. The largest inhibition zone diameter among the treatment groups was observed at a concentration of 0.8%, measuring 10.25 ± 0.95 mm, while the positive control produced an inhibition zone diameter of 11.25 ± 0.95 mm.
The results of the one-way ANOVA test showed a significant difference among the treatment groups (p = 0.049). Supplementation with ethanol extract of wood ear mushrooms did not result in higher bacteriocin antimicrobial activity compared to the positive control.

Keywords: A. auricula-judae, bacteriocin, L. plantarum, S. typhimurium, inhibition zone.
Literature: 36 2015 - 2025