Infeksi virus hepatitis B (VHB) tetap menjadi tantangan kesehatan
masyarakat yang dapat dicegah melalui vaksinasi, dengan keberhasilan ditandai
oleh pembentukan kadar antibodi anti-HBs protektif (>10 mIU/mL). Namun,
respons imun humoral pascavaksinasi bervariasi karena dipengaruhi faktor internal
seperti Indeks Massa Tubuh (IMT) serta kondisi psikologis seperti stres akademik.
Penelitian kuantitatif observasional analitik dengan pendekatan cross
sectional ini bertujuan untuk mengetahui hubungan antara nilai IMT dan tingkat
stres akademik dengan titer antibodi hepatitis B pada 40 mahasiswa pascavaksinasi
di Universitas Jenderal Achmad Yani, Cimahi, yang dipilih menggunakan teknik
purposive sampling. Pengukuran IMT dilakukan melalui penimbangan berat badan
dan tinggi badan, tingkat stres diukur menggunakan kuesioner Lakaev Academic
Stress Response Scale (LASRS), sedangkan titer anti-HBs diperiksa dengan metode
Enzyme Linked Immunosorbent Assay (ELISA) sandwich menggunakan kit reagen
yang memiliki sensitivitas 100

Hepatitis B virus (HBV) infection remains a serious public health challenge
that is preventable through vaccination, with success indicated by the formation of
protective anti-HBs levels (>10 mIU/mL). However, the post-vaccination humoral
immune response varies, as it is influenced by internal factors such as Body Mass
Index (BMI) and academic stress.
This quantitative, analytical observational study with a cross-sectional
approach aimed to determine the relationship of BMI and academic stress levels
with hepatitis B antibody titers among 40 post-vaccinated students at Universitas
Jenderal Achmad Yani, Cimahi, selected via purposive sampling. BMI data were
obtained by measuring weight and height, stress levels were measured using the
Lakaev Academic Stress Response Scale (LASRS) questionnaire, and anti-HBs
titers were examined using the sandwich ELISA method sensitivity 100% and
specificity 99% at the Immunology Laboratory of FITKes Unjani. Data were
analyzed using descriptive statistics and Spearman’s Rho bivariate correlation test.
The univariate analysis showed that the majority of respondents had a low BMI
(62.5%), experienced moderate (47.5%) to high (37.5%) academic stress, yet
maintained protective immunity (70%). The bivariate test revealed no significant
correlation between BMI and anti-HBs titers (p=0.872; r=0.026) nor between
academic stress levels and anti-HBs titers (p=0.431; r=0.128). In conclusion, BMI
and academic stress are not significantly correlated with the maintenance of anti
HBs titers in post-vaccinated students, suggesting long-term immunity is more
likely determined by immunological memory.
Future studies are recommended to utilize a larger sample size and consider
other external factors.
Keywords: Anti-HBs, ELISA, Body Mass Index, Academic Stress